| Ex (nm) | 526 | Em (nm) | 543 |
| 分子量 | N/A | 溶剂 | Water |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品基本信息
产品名称：VIC 染料 qPCR 校准溶液 *10,000X*
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
分子量：NA
溶剂：水
激发波长(nm)：526
发射波长(nm)：543
产品介绍
实时热循环仪需要校准才能生成荧光信号的光谱图。由于仪器的荧光检测灵敏度可能不同，校准任何染料（如 VIC）所需的浓度将取决于所使用的设备。 AAT Bioquest VIC 染料 qPCR 校准溶液以 10,000X 浓缩液形式提供，可与多种 qPCR 仪器兼容，包括 ABI7500 Fast、QuantStudio™ 和 ViiA™ 7 系统。校准和验证应每六个月或根据需要运行一次。百萤生物是AAT Bioquest的中国代理商，为您提供优质的VIC 染料 qPCR 校准溶液 *10,000X*。
实验方案
此通用协议适用于 ABI7500 FAST qPCR 仪器。
准备 VIC 校准板：
1.将 1 μL 的 10,000X VIC 染料 qPCR 校准溶液与 10 mL 的无核酸酶水或 qPCR 缓冲液混合，制备 VIC 染料 qPCR 校准工作溶液。请注意，染料浓度必须根据仪器的校准要求进行优化。
2.在反应板的所有孔中加入 20 μL 的 VIC Dye qPCR 校准工作溶液。
3.用光学胶盖密封板
4.以 < 1500 rpm 的速度将板离心 2 分钟
5.检查板的每个孔中的液体是否位于孔的底部。如果没有，请再次以稍高的 rpm 和更长的时间将板离心
6.有关完整的校准说明，请参考您仪器的维护指南
参考文献
Authors: Xiang, Jing and Wang, Xingmin and Ding, Mingmei and Tang, Xiaomin and Zhang, Shixin and Zhang, Xianming and Xie, Zongli
Journal: Chemosphere (2022): 133728
Authors: Lin, Lina and Jiang, Tiancheng and Liang, Yonghong and Pervez, Md Nahid and Navik, Rahul and Gao, Bo and Cai, Yingjie and Hassan, Mohammad Mahbubul and Kumari, Naveeta and Naddeo, Vincenzo
Journal: Materials (basel, Switzerland) (2022)
Authors: Shelke, Harshad D and Machale, Archana R and Survase, Avinash A and Pathan, Habib M and Lokhande, Chandrakant D and Lokhande, Abhishek C and Shaikh, Shoyebmohamad F and Rana, Abu Ul Hassan S and Palaniswami, Marimuthu
Journal: Materials (basel, Switzerland) (2022)
Authors: Okada, Mali and Egan, Catherine A and Heeren, Tjebo Fc and Valmaggia, Philippe and Tufail, Adnan and Maloca, Peter M
Journal: American journal of ophthalmology case reports (2022): 101267
Authors: Park, Inkyung and Oh, Sungtaek and Nam, Hyoyoun and Celi, Pietro and Lillehoj, Hyun S
Journal: Poultry science (2022): 101731
Authors: Clark, Michael and Tepper, Kate and Petroll, Kerstin and Kumar, Sheemal and Sunna, Anwar and Maselko, Maciej
Journal: ACS synthetic biology (2022): 308-316
Authors: Liu, Wei and Huang, Jie and Weatherley, Anthony J and Zhai, Wenjun and Liu, Fuya and Ma, Zhiling and Jiao, Yunhong and Zhang, Chao and Han, Bing
Journal: Journal of environmental management (2022): 113862
Authors: Floyd, Thomas G and Song, Ji-Inn and Hapeshi, Alexia and Laroque, Sophie and Hartlieb, Matthias and Perrier, Sébastien
Journal: Journal of materials chemistry. B (2022): 3696-3704
Authors: Knezic, Adriana and Broughton, Brad R S and Widdop, Robert E and McCarthy, Claudia A
Journal: Scientific reports (2022): 7598
Authors: Mehta, Sourabh and Bongcaron, Viktoria and Nguyen, Tien K and Jirwanka, Yugandhara and Maluenda, Ana and Walsh, Aidan P G and Palasubramaniam, Jathushan and Hulett, Mark D and Srivastava, Rohit and Bobik, Alex and Wang, Xiaowei and Peter, Karlheinz
Journal: Small (Weinheim an der Bergstrasse, Germany) (2022): e2200967
