Cell Meter Annexin V凋亡检测试剂盒是美国AAT Bioquest生产的细胞凋亡检测试剂盒，我们的Cell Meter 检测试剂盒是一套用于检测细胞生存力的工具。有多种参数可用于检测细胞活力。该特定试剂盒旨在通过测量磷脂酰丝氨酸（PS）的转运来监测细胞凋亡。
数据分析
在活的非凋亡细胞中，膜联蛋白V-iFluor 488检测非诱导细胞中的先天性细胞凋亡，其通常为所有细胞的2-6％。 在凋亡细胞中，膜联蛋白V-iFluor 488与磷脂酰丝氨酸结合，磷脂酰丝氨酸位于细胞膜的外部小叶上，因此导致染色强度增加。
参考文献
Combined Treatments of Magnetic Intra-Lysosomal Hyperthermia with Doxorubicin Promotes Synergistic Anti-Tumoral Activity.
In situ polymerizable hydrogel incorporated with specific pathogen-free porcine platelet-rich plasma for the reconstruction of the corneal endothelium
Novel regulations of MEF2-A, MEF2-D, and CACNA1S in the functional incompetence of adipose-derived mesenchymal stem cells by induced indoxyl sulfate in chronic kidney disease
Shear stress-induced alteration of epithelial organization in human renal tubular cells
Targeting a G-protein-coupled receptor overexpressed in endocrine tumors by magnetic nanoparticles to induce cell death
公司简介
图1.检测膜联蛋白V-iFluor 488和磷脂酰丝氨酸在Jurkat细胞中的结合活性。 Jurkat细胞在37℃，5％CO2培养箱中不用（蓝色）或用20μM喜树J（红色）处理4-5小时，然后用Annexin V-iFluor 488染色30分钟。 使用FL1通道用FACSCalibur（Becton Dickinson）流式细胞仪测量膜联蛋白V-iFluor 488的荧光强度。
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西安百萤生物科技有限公司是一家专注从事生物、化学试剂及其试剂盒的高新技术企业；主要以荧光探针，分子探针，核酸/蛋白质标记、细胞检测等技术和产品为研发核心，产品应用于基因合成、细胞检测、活体示踪，抗体标记、新药筛选等领域。
