ATCC16528 Prototheca stagnora Cooke
北京百欧博伟生物技术有限公司（biobw）是北京的一家专业于生物技术的研究与广泛运用及推广的高科技公司，位于北京企业林立的丰台区造甲街，生物技术推广及运用早于2011年开始，成立公司于2013年一月。本公司主要提供色谱耗材、色谱仪器、固相萃取装置、化学试剂、样品瓶、氘灯、标准品、微生物技术、菌种保藏服务以及ATCC产品代理等产品及服务。
平台编号：bio-103873
规格：frozen
拉丁属名：Prototheca stagnora Cooke
购买数量： 1
Prototheca stagnora Cooke 拉丁名
ATCC® 16528 编号
Strain Designations 菌株别名 SEC-L-1690 [UTEX 1443]
Biosafety Level 安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation 分离基物 ludge, Lebanon, OH, 1961
Product Format 提供形式 frozen
Type Strain 模式菌株 yes
Medium 培养基 ATCC® Medium 28: Emmons' modification of Sabouraud's agar
Growth Conditions 生长条件
Temperature 培养温度 : 25.0°C
Duration: axenic
Protocol: ATCCNO: 16522 SPEC: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium to containing 12% sucrose to the freeze-dried inner shell vial. once completely rehydrated, aseptically transfer the material to a 100 mm agar plate of the appropriate medium and evenly distribute the material over the surface of the agar with a spread bar. Subculture 4-6 weeks when incubated at 25C. Subculture 6-12 months when incubated at 18C To subculture, transfer a loopful of material to a fresh plate and spread evenly over the surface.
Subcultivation
Protocol: ATCCNO: 16522 SPEC: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium to containing 12% sucrose to the freeze-dried inner shell vial. once completely rehydrated, aseptically transfer the material to a 100 mm agar plate of the appropriate medium and evenly distribute the material over the surface of the agar with a spread bar. Subculture 4-6 weeks when incubated at 25C. Subculture 6-12 months when incubated at 18C To subculture, transfer a loopful of material to a fresh plate and spread evenly over the surface.
Cryopreservation
1. Harvest cells from a culture which is at or near peak density by adding 3.0-5.0 ml fresh ATCC medium 28 broth to the slant or plate and washing cells into suspension. It may be helpful to rub the surface of the agar with a spread bar or inoculating loop to detach adhering cells.
2. Adjust the concentration of cells to 2 x 107/ml with fresh broth medium, then dilute to half this concentration by adding an equal amount of a 20% (v) sterile solution of either DMSO or glycerol in fresh ATCC medium 28 broth.
3. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). The time from mixing of the cell preparation and the cryoprotective solution to the start of the cooling cycle should be no less than 15 min and no greater than 30 min.
4. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
5. The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials can be stored between -80 and -70°C for no longer than one week.
6. To establish a culture from the frozen state place an ampule in a water bath set at 35°C until thawed (2-3 min). Immerse the ampule enough to cover only the frozen material. Do not agitate the ampule.
7. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and add to
a fresh slant of ATCC medium 28 or the surface of an agar plate of ATCC medium 28.
8. Maintain as described above.
Name of Depositor WB Cooke
Year of Origin 1961
References 参考文献
Pore RS. Prototheca taxonomy. Mycopathologia 90: 129-139, 1985.
Cooke. J. Elisha Mitchell Sci. Soc. 84: 217-220, 1968.
type strain
欢迎访问北京百欧博伟生物技术有限公司网站，本站隶属于北京百欧博伟生物技术有限公司，单位现提供微生物菌种及其细胞等相关产品查询、咨询、订购、售后服务！与国内外多家研制单位，生物医药，第三方检测机构，科研院所有着良好稳定的长期合作关系！欢迎广大客户来询！
